Additionally, the toxR gene was incorporated into the assay for the confirmation of V. Through extensive sequence analyses using 418 genomes, specific primers and probes were designed for a total of 22 antigen gene targets for the O- and K- serogroups. parahaemolyticus serotypes spanning a 10-year period. We describe the development of a multiplex ligation reaction based on probe melting curve analysis (MLMA) for the simultaneous identification of 11 clinically most common V. parahaemolyticus strains to improve disease surveillance and outbreak investigations. Despite the continued rise of disease incidence and outbreaks of vibriosis, as well as the global emergence of pandemic clones and serovariants with enhanced virulence, there is a paucity of molecular methods for the serotyping of V. The dynamic nature of Vibrio parahaemolyticus epidemiology has presented a unique challenge for disease intervention strategies.
1College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.Minxu Li 1, Yixiang Jiang 2, Xiaolu Shi 2, Yinghui Li 2, Min Jiang 2, Yiman Lin 2, Yaqun Qiu 2, Le Zuo 2, Yinhua Deng 3, Zeren Lin 1, Yiqun Liao 4, Qingge Li 4 and Qinghua Hu 1,2 *
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